Genetics Course Page Fall 2019

Minot State University

Genetics/ Biol 215

Course Page

Fall 2019

 

Announcements
 
Check out this advance in the treatment of Tay-Sachs disease.
 
Another recessive human genetic disease with huge advance in treatment--Check out new drug development for Cystic Fibrosis
 
 
 
 
 
Point total (updated November 7th) = 231 points
 
 

Lectures

Lecture 1

Lecture 2

Lecture 3     CRISPR and Sickle Cell Anemia

Lecture 4     More on Cell cycle control

Lecture 5

Lecture 6

Lecture 7.     Paper --Down syndrome.         Tumor suppressor  in DSCR

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Lecture 8

Lecture 9

Lecture 10

Lecture 11--INDEPENDENT STUDY FOR 9-27-19

Lecture 12

Lecture 13

Lecture 14

Lecture 15

Lecture 16

Lecture 17

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Lecture 18

Lecture 19

Lecture 20

Lecture 21

Lecture 22

Lecture 23

Lecture 24

Lecture 25

Lecture 26

Lecture 27

Novembern 8th, 2019---We will just finigh the concepts of lecture 27, go over lab quiz 2 and look at transformation plates.  

Lab 8--Observations and discussion.

Lecture 28

Lecture 29

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Lecture 30

Lecture 31--Few new slides from last lecture

Lecture 32

Lecture 33

 

Lab

Lab Manual--2019

lab report guidelines

Lab 1--preparation of onion root

Lab 1--Handouts for Sept. 5th, 2019

Example lab report  Do not plagiarize. This is not a perfect report.   *This example report was written before students participated in the growing of their own roots.  Don't forget that part of the protocol. 

Print both below for Lab 2

Lab 2---Meiosis Part 1

Lab 2---Meiosis Part 2

MEIOSIS POWERPOINT CLICK HERE 

Lab 3--Mendel Part 1

Lab 4--Analyisis of corn data    Compiled Data here (2 different sheets to spreadsheet).  2-page pdf hard copy of data: HERE

Guidelines for lab 3-4

Lab 5--Introduction to Drosophila and X-linkage. Introductory lecture

Lab 6--Human traits (We will also set up Drosophila crosses today)

Lab 7---Analyisis of human traits---Population genetics.  Hardy-Weinberg. Class data here:

Lab report 5/7 guidelines

Lab 8---Bacterial Transformation--DNA is the genetic material. Lab 8--Observations and discussion.

Lab 9---Finish up bacterial transformation, discuss GFP and basics of gene regulation AND analyze Drosophila F1 and introduce 3-point mapping.-- New lab materials provided in lab.   Bring this:Observations and discussion.

Presentation on 3 point mapping

Presentation on gene regulation (GFP)

Lab 10--Polymerase Chain Reaction--determining your PV92 genotype/molecular genotyping   

PCR LECTURE

PCR  (Animation)

 

 

 

Lecture Documents/Assigned Reading

Syllabus  Fall 2019

lecture summary page

 

Exam Prep

Topics lists

Exam 1 topics list

Exam 2 topics list

Exam 3 topics list

 

Practice Problems

Problem Set 1    Key/Solutions

Problem Set 2.    Key/Solutions

Pedigree problems--including X-linkage  Hemophilia story--questions

Problem Set 3     Key/solutions

Problem Set 4--linkage/recombination mapping.   Key/Solutions

 

Animations

Mitosis

Meiosis

 

Below this line---Ignore!  Old material

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Replication 1

Replication 2

 

Problem set 3---Many similar practice problems in the text---try colored boxes in the chapters.   

 

             

 

 

 

  

Lab 9--Determining you genotype for PV92 "gene".  Polymerase Chain Reaction. (Lab handout says lab 10).   PCR LECTURE

Drosophila F2 data analysis--guidelines

Lab 9 part 2---analysis of PCR products.  It is essential that you are prepared for this lab.  Read your lab handout from Lab 10- Part 1 to refresh your memory of PCR and to begin to understand the dimorphic nature of the PV92 locus.  PV92 has 2 alleles. Predict  your genotype.  Review animation on electrophoresis. 

Electrophoresis animation: http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

PV92 gels here.  9 am lab     1 pm lab

 

 

 HERE

 

 

Paper 1---Genetically engineering animals for human health

 

Paper 3 Dog gentetics paper

Paper 4--Variation in size in dogs--read abstract.

Paper 5---X-inactivation molecular mechanism

 

Lab 8--Bacterial transformation "seeing the genetic material at work"  Transformation discussion--Here

Lab 9---Determinining your PV92 genotype---PCR and genetic polymorphisms.  PCR LECTURE

Lab 7---take home lab on Polygenic/Continuous traits

Lab 8---Drosophila part II and Introduction to recombination mapping Part I and Part II.

Lab 9---Bacterial transformation---"seeing" the genetic material.

Lab 10 Transformation data and  discussion questions  AND Drosophila Part III 

  

 

http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

100 bp ladder

1 kb ladder

 

View Animation---Transcription (prokaryotes) and more Transcription (eukaryotes)

 

Protein Synthesis Animation--translation

animations---translation

Electrophoresis animation: http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

 

 

 

Ch. 2 multiple choice

 

 

 

 

 

 

 

 

 

 

 

 

 

Guidelines for Lab 6-7 report

Lab 8---  We will be studying a polygenic/quantitative human trait related to fingerprints.

Lab 9---Molecular genetics--Bacterial transformation  After overnight incubation please use this handout to observe your plates.  

Lab 10--Polymerase Chain Reaction--determining your PV92 genotype

 

 

PCR

 

 

 

 

  

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AttachmentSize
National Geo dog genetics story.pdf3.66 MB
problem set 3.pdf54.9 KB
Lecture 30-2018 Fall.ppt2.91 MB
Lecture 31-2018 Fall.ppt3.11 MB
Lecture 32-2018 Fall.ppt2.37 MB
Lecture 33-2018 Fall.ppt2.46 MB
Lecture 34-2018 Fall.ppt2.7 MB
Lecture 33-2018 Fall.ppt2.46 MB